Fig. 4From: Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analysesCRTC3 overexpression induces gene programs involved in metabolism. The CON and OE adipocytes were harvested at 48 h after treatment, and RNA was purified for RNA-seq. a. Volcano plot of the altered genes in OE versus CON adipocytes. The threshold (|log2-fold change| > log2 1.5; padj< 0.05) was defined as a significant change. b. The upregulated genes were analyzed by performing a statistical analysis of the KEGG pathway enrichment scatter plot (top 20 genes). The triangle size indicates the significance and the corresponding significance values are displayed as log10 (P-value). c. The downregulated genes were analyzed using the KEGG pathway enrichment statistical scatter plot (top 20 genes). d, e. Heatmap of relative expression of selected T2D mellitus- (d) and lipolysis-related (e) genes from the RNA-seq dataset. f. GO enrichment analysis (top 10 genes). CON represents the control group, and OE represents the CRTC3 overexpression groupBack to article page