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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Comprehensive evaluation of the metabolic effects of porcine CRTC3 overexpression on subcutaneous adipocytes with metabolomic and transcriptomic analyses

Fig. 4

CRTC3 overexpression induces gene programs involved in metabolism. The CON and OE adipocytes were harvested at 48 h after treatment, and RNA was purified for RNA-seq. a. Volcano plot of the altered genes in OE versus CON adipocytes. The threshold (|log2-fold change| > log2 1.5; padj< 0.05) was defined as a significant change. b. The upregulated genes were analyzed by performing a statistical analysis of the KEGG pathway enrichment scatter plot (top 20 genes). The triangle size indicates the significance and the corresponding significance values are displayed as log10 (P-value). c. The downregulated genes were analyzed using the KEGG pathway enrichment statistical scatter plot (top 20 genes). d, e. Heatmap of relative expression of selected T2D mellitus- (d) and lipolysis-related (e) genes from the RNA-seq dataset. f. GO enrichment analysis (top 10 genes). CON represents the control group, and OE represents the CRTC3 overexpression group

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