Fig. 3
CRTC3 overexpression alters the overall composition of metabolites. The CON and OE adipocytes were harvested at 48 h after treatment, and analyzed using LC-MS/MS. a. Volcano plot of pairwise comparisons of all detected ions. The threshold (|log2-fold change| > log2 1; P value< 0.05) was defined for each significantly changed metabolites. b. Significantly altered pathways based on the enrichment and topology analyses. The transverse and vertical dotted lines indicate the value for pathway enrichment and pathway impact, respectivly. Each dot represents a metabolic pathway, and the dot size indicates the significance. c. The composition of the significantly altered metabolites. d. The altered lipid metabolites in CRTC3-overexpressing adipocytes. e. The subclasses of the significantly altered lipid metabolites. FAs, fatty acyls; GP, glycerophospholipids; SP, sphingolipids; ST, steroids; FAC, Fatty acyl carnitines; PL, prenol lipids