Fig. 4

Analysis of the differential metabolites between the HFSS and LFSS groups. a Identification of differentially expressed metabolites (DEMs) between the HFSS and LFSS groups. The outer circle displays the P values of One-way ANOVA, where P values are plotted as −ln (P). The inner circle shows variable importance in projection (VIP) values of the OPLS-DA model. Each point represents a metabolite and the blue dashed line shows the significance threshold (P < 0.05 or VIP > 1). The gray dashed line indicates the P value, and the VIP of one metabolite all passed the significance threshold. b Heatmap of the 28 significantly different metabolites between the HFSS and LFSS hens. The heatmap is color-coded based on row z-scores. The corresponding metabolites of the Arabic numerals are soared by P-value. c Pearson correlation analysis of the 28 DEMs. The corresponding metabolites of the Arabic numerals are the same as shown in Fig. 4b and Table 2. The color of the circles indicates the magnitude of correlation between metabolites, and the symbol * indicates that a P < 0.05. d Metabolic pathway identification of DEMs. The corresponding enriched pathways are displayed in Additional file 6: Table S4