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Table 5 Differential metabolites that mapped to pathway on day 3 post-hatch

From: Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

Differential metabolites

Retention time

m/z

Fold changea

P-valueb

VIPc

Pathway

Stachyose

0.6476833

701.18808

0.420

0.007

3.454

Galactose metabolism

Raffinose

0.6476833

539.13622

0.555

0.004

2.501

Manninotriose

0.6476833

539.13622

0.555

0.004

2.501

Sucrose

0.6334000

377.08445

0.605

0.002

2.146

Taurine

0.5905500

124.00700

1.577

0.005

1.810

Primary bile acid biosynthesis

Taurochenodeoxycholate

7.2767833

498.28747

0.283

0.004

4.431

Taurodeoxycholate

8.2390167

498.28884

0.400

0.007

3.579

Tauroursodeoxycholic acid

6.9417667

498.28752

0.444

0.030

2.550

Linoleic acid

8.6883500

279.23228

0.796

0.042

1.408

Linoleic acid metabolism

Dihomo-γ-linolenic acid

9.5298333

305.24812

1.414

0.047

1.141

Linoleic acid metabolism

9,10-DHOA

8.4390333

313.23741

2.468

0.027

3.496

Linoleic acid metabolism

12,13-DHOA

7.5975333

313.23730

0.606

0.039

1.442

Linoleic acid metabolism

13,16,19-Docosatrienoic acid

9.8934500

333.27922

4.063

0.038

3.999

Lipid metabolism

DHA ethyl ester

9.5298333

355.26264

1.771

0.034

1.592

Amaranthin

0.6334000

761.14313

0.384

0.005

3.363

Secondary metabolites

Cucurbitacin D

9.4012667

515.30267

0.339

0.011

4.030

  1. aFold Change in the Arg10 group compared with the NC group. bP-values were calculated from Kruskal-Wallis test. cVIP (variable importance in the projection) values were obtained from partial least squares discriminant analysis models