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Table 3 Effects of in ovo feeding of L-arginine on digestive organ index of layer chicks1

From: Dynamic alterations in early intestinal development, microbiota and metabolome induced by in ovo feeding of L-arginine in a layer chick model

Item2  Arg dosage (mg/egg)Pooled SEMP-value
NCSC2610ANOVALinear3Quadratic3
d 0 Index, %
 Yolk sac14.52a14.49a11.05b11.72b11.94b0.400.0080.5510.282
 Proventriculus0.530.560.660.540.600.020.0860.6560.910
 Gizzard4.014.354.484.234.530.080.2110.3520.800
 Duodenum16.3415.7015.5615.7716.150.200.7210.2940.602
 Jejunum28.16b28.68b34.49a29.61b31.79ab0.730.0200.9280.841
 Ileum25.4025.5426.3425.4025.990.640.9890.9100.896
d 3 Index, %
 Yolk sac2.672.911.931.891.850.160.0880.0650.159
 Proventriculus1.161.171.181.231.210.020.6250.8570.204
 Gizzard6.526.256.546.626.570.070.5360.6110.207
 Duodenum22.48b22.67b22.93ab24.71ab25.39a0.400.0490.0100.035
 Jejunum42.1341.0741.7344.7042.610.570.3230.4670.112
 Ileum31.2332.9834.7536.3633.920.740.2240.8030.157
d 14 Index, %
 Proventriculus0.770.710.760.800.740.010.1000.5460.131
 Gizzard3.633.573.683.623.660.050.9710.6740.924
 Duodenum10.7010.659.399.2210.710.400.6060.8220.272
 Jejunum19.02b19.21b22.52a22.05a23.66a0.450.0010.0250.039
 Ileum18.5617.5820.0719.9420.930.420.0890.0400.075
d 21 Index, %
 Proventriculus0.710.670.690.710.680.010.4660.5250.284
 Gizzard2.992.842.742.912.950.040.3650.7410.879
 Duodenum7.827.737.387.677.510.080.5030.7020.975
 Jejunum15.3716.3615.1016.2114.240.270.1080.5470.651
 Ileum14.5115.9214.5415.1713.240.300.1090.7770.305
d 42 Index, %
 Proventriculus0.690.710.630.720.710.020.3080.4220.578
 Gizzard2.602.472.442.572.430.050.7400.8660.701
 Duodenum4.133.853.884.103.970.050.1940.3280.229
 Jejunum8.43b7.89b7.77b8.04b9.21a0.130.0010.0310.042
 Ileum7.72a7.17ab6.58b7.54a7.80a0.120.0040.2350.103
  1. a–bMeans within a row with no common superscripts differ significantly (P < 0.05). 1 Data are the mean of 8 replicates. NC, non-injected control group; SC, saline-injected control group.2 Yolk sac and stomach index, % = absolute weight, g / body weight, g × 100, Intestinal index, % = absolute length, cm/ body weight, g × 100. 3Orthogonal polynomial contrasts were used to determine the linear and quadratic effects of increasing concentrations of L-arginine solution