Items | Experimental treatments | SEM | P-value | ||
---|---|---|---|---|---|
H (n = 20) | SM (n = 20) | CM (n = 20) | |||
Lactation parameters | |||||
 Milk yield, kg/d | 42.6a | 32.7b | 28.5b | 1.43 | < 0.010 |
 Milk fat, % | 3.58a | 2.80b | 2.14c | 0.14 | < 0.010 |
 Milk protein, % | 3.10b | 3.06b | 3.42a | 0.10 | < 0.010 |
 Lactose, % | 4.92a | 3.95b | 2.57c | 0.18 | < 0.010 |
 Milk SCC, ×103 cells/mL | 42.8c | 1002b | 7053a | 409 | < 0.010 |
Rumen fermentation parameters | |||||
 pH | 6.68 | 6.71 | 6.73 | 0.03 | 0.214 |
 NH3-N, mg/dL | 10.5 | 8.64 | 8.40 | 0.40 | 0.061 |
 RUN, mg/dL | 5.90 | 5.73 | 6.02 | 0.20 | 0.851 |
 LA, mmol/L | 0.86b | 0.90a | 0.80c | 0.01 | < 0.010 |
 Acetate, mmol/L | 64.5a | 60.1a | 41.0b | 1.67 | < 0.010 |
 Propionate, mmol/L | 27.7a | 25.2a | 19.2b | 0.87 | < 0.010 |
 A/P | 2.63 | 2.35 | 2.45 | 0.06 | 0.111 |
 Isobutyrate, mmol/L | 0.91 | 0.85 | 0.83 | 0.02 | 0.325 |
 Butyrate, mmol/L | 12.1a | 12.1a | 8.36b | 0.36 | < 0.001 |
 Isovalerate, mmol/L | 1.58 | 1.46 | 1.38 | 0.05 | 0.213 |
 Valerate, mmol/L | 1.54a | 1.55a | 1.15b | 0.05 | < 0.010 |
 TVFAs, mmol/L | 109a | 105a | 76.2b | 3.00 | < 0.010 |