Fig. 5

Significantly differential metabolites (top 15) in the rumen of dairy cows between a CM and H cows, b SM and H cows and c CM and SM cows based on variable importance in the projection (VIP) value under merger mode of positive and negative ion. H, healthy; SM, subclinical mastitis; CM, clinical mastitis. Each column represents a sample, each row represents a metabolite, and the color indicates the relative expression level of the metabolites in the samples. The right side is the metabolite VIP bar graph, the length of bar represents the contribution of the metabolites to the difference between the two groups (VIP > 1), the color of the bar means the FDR-adjusted P-value (*0.01 < FDR-adjusted P ≤ 0.05; **0.001 < FDR-adjusted P ≤ 0.01; *** FDR-adjusted P ≤ 0.001)