Table 3 Association between haplotype blocks in AGPAT3 and milk fatty acid traits in Chinese Holstein cows (LSM ± SE)

Block 1

H1H1(121–130)

0.3692 ± 0.0166^A

0.8989 ± 0.0140^ACa

2.7932 ± 0.0413^a

0.0579 ± 0.0041

2.9643 ± 0.0538

0.1020 ± 0.0043

10.1573 ± 0.0917

0.6359 ± 0.0253

0.9978 ± 0.0175

34.9164 ± 0.2400

1.3602 ± 0.0336

0.5739 ± 0.0044

H1H2(218–240)

0.4422 ± 0.0142^B

0.8923 ± 0.0122^Aa

2.7610 ± 0.0367^A

0.0569 ± 0.0038

2.9761 ± 0.0470

0.0983 ± 0.0035

10.2724 ± 0.0804

0.6296 ± 0.0213

0.9938 ± 0.0149

34.8598 ± 0.2082

1.3269 ± 0.0292

0.5767 ± 0.0037

H1H3(191–204)

0.5563 ± 0.0146^C

1.0162 ± 0.0128^Bb

2.9065 ± 0.0373^Bb

0.0596 ± 0.0039

3.0334 ± 0.0487

0.1005 ± 0.0037

10.3997 ± 0.0839^a

0.6786 ± 0.0225

1.0068 ± 0.0153

34.7483 ± 0.2175

1.3176 ± 0.0303

0.5656 ± 0.0039

H2H2(98–103)

0.4381 ± 0.0175^B

0.9044 ± 0.0152^ACDac

2.8120 ± 0.0436

0.0594 ± 0.0043

2.9978 ± 0.0575

0.0987 ± 0.0046

10.3001 ± 0.0986

0.6575 ± 0.0274

0.9853 ± 0.0190

34.7264 ± 0.2591

1.2820 ± 0.0363

0.5686 ± 0.0048

H2H3(136–150)

0.4610 ± 0.0157^B

0.9441 ± 0.0134^CDcd

2.7551 ± 0.0400^A

0.0575 ± 0.0040

2.9299 ± 0.0521

0.0956 ± 0.0041

10.1961 ± 0.0869

0.6483 ± 0.0243

0.9835 ± 0.0166

35.0655 ± 0.2324

1.3081 ± 0.0326

0.5694 ± 0.0042

H3H3(66–72)

0.5532 ± 0.0206^C

0.9642 ± 0.0177^BDd

2.8116 ± 0.0513

0.0597 ± 0.0048

3.0030 ± 0.0657

0.0978 ± 0.0056

10.0632 ± 0.1132^b

0.6629 ± 0.0329

0.9895 ± 0.0227

34.8421 ± 0.3116

1.3749 ± 0.0428

0.5689 ± 0.0056

P

<.0001**

<.0001**

0.0001**

0.9269

0.4138

0.8544

0.0105*

0.3696

0.8195

0.8036

0.3084

0.0962

Block 2

H1H2(126–139)

0.5694 ± 0.0167^Aa

0.9932 ± 0.0145^Aa

2.8297 ± 0.0425^a

0.0580 ± 0.0042

3.0485 ± 0.0549

0.0962 ± 0.0043

10.1137 ± 0.0946

0.6361 ± 0.0253

0.9918 ± 0.0176

34.9810 ± 0.2481

1.3447 ± 0.0344

0.5703 ± 0.0045

H1H3(56–62)

0.6103 ± 0.0213^Da

1.0604 ± 0.0180^B

2.8139 ± 0.0519

0.0575 ± 0.0050

2.9637 ± 0.0688

0.0984 ± 0.0057

9.9868 ± 0.1182

0.6793 ± 0.0335

0.9893 ± 0.0231

34.7801 ± 0.3163

1.3754 ± 0.0439

0.5671 ± 0.0058

H1H4(52–55)

0.4357 ± 0.0224^Bbc

0.9280 ± 0.0192^ACd

2.8422 ± 0.0552

0.0631 ± 0.0053

3.0676 ± 0.0718

0.1029 ± 0.0061

10.3528 ± 0.1232

0.6664 ± 0.0353

1.0077 ± 0.0243

34.2739 ± 0.3353

1.4103 ± 0.0465

0.5652 ± 0.0062

H2H2(138–149)

0.3787 ± 0.0166^Cbe

0.8735 ± 0.0140^Cde

2.7289 ± 0.0417

0.0579 ± 0.0042

2.9456 ± 0.0546

0.0967 ± 0.0042

10.0997 ± 0.0927

0.6586 ± 0.0250

0.9760 ± 0.0171

34.7218 ± 0.2397

1.3455 ± 0.0335

0.5744 ± 0.0043

H2H3(95–102)

0.5078 ± 0.0181^Ac

0.8930 ± 0.0155^Cbd

2.6966 ± 0.0455

0.0611 ± 0.0045

2.9215 ± 0.0594

0.0950 ± 0.0048

10.0970 ± 0.1008

0.6550 ± 0.0283

0.9821 ± 0.0192

35.2877 ± 0.2714

1.3358 ± 0.0376

0.5593 ± 0.0049

H2H4(96–107)

0.4629 ± 0.0187^Fc

0.8165 ± 0.0155^Dc

2.6801 ± 0.0452^b

0.0580 ± 0.0045

2.9780 ± 0.0594

0.1005 ± 0.0048

10.3642 ± 0.1024

0.6403 ± 0.0284

0.9990 ± 0.0193

34.9866 ± 0.2709

1.3292 ± 0.0373

0.5769 ± 0.0048

H2H5(63–68)

0.3384 ± 0.0204^Ede

0.8526 ± 0.0179^Cbce

2.7312 ± 0.0510

0.0575 ± 0.0049

2.9783 ± 0.0671

0.0982 ± 0.0056

10.0688 ± 0.1129

0.6521 ± 0.0319

0.9807 ± 0.0223

34.6563 ± 0.3081

1.4043 ± 0.0426

0.5783 ± 0.0056

P

<.0001**

<.0001**

0.0013**

0.8887

0.2555

0.9225

0.0127*

0.9124

0.8845

0.1205

0.4760

0.0193*

Haplotype block

Haplotype combination (No.)

C17:1, %

C18:0, %

C18:1cis-9, %

C18index, %

C20:0, %

C14index, %

C16index, %

C17index, %

SFA, %

UFA, %

SFA/UFA

Total index, %

Block 1

H1H1(105–130)

0.1992 ± 0.0034

14.1901 ± 0.1259

19.3463 ± 0.1683

57.4699 ± 0.3816

0.1791 ± 0.0025^Aad

6.0996 ± 0.1885

3.7792 ± 0.0860

25.0181 ± 0.2938

67.7674 ± 0.2297

30.6829 ± 0.2089

2.2565 ± 0.0304

27.6878 ± 0.1931^a

H1H2(193–241)

0.1904 ± 0.0029

14.3563 ± 0.1050^a

19.1954 ± 0.1414

56.9474 ± 0.3225

0.1710 ± 0.0020^ABc

5.9684 ± 0.1634

3.6878 ± 0.0747

24.6437 ± 0.2483

67.9734 ± 0.1925

30.4159 ± 0.1766

2.2675 ± 0.0255

27.5081 ± 0.1649

H1H3(167–204)

0.1926 ± 0.0030

13.9560 ± 0.1103^b

19.0385 ± 0.1501

57.3701 ± 0.3377

0.1635 ± 0.0022^Bb

6.3161 ± 0.1697

3.6750 ± 0.0774

24.9275 ± 0.2620

68.0604 ± 0.2014

30.1895 ± 0.1868

2.2838 ± 0.0269

27.4224 ± 0.1743

H2H2(93–103)

0.1912 ± 0.0036

14.0036 ± 0.1391

19.2225 ± 0.1833

57.5431 ± 0.4143

0.1731 ± 0.0025^Aac

6.1465 ± 0.2072

3.5740 ± 0.0928

24.2275 ± 0.3182^a

67.9786 ± 0.2486

30.3912 ± 0.2271

2.2608 ± 0.0330

27.6407 ± 0.2079

H2H3(119–150)

0.1922 ± 0.0032

14.2354 ± 0.1206

18.8881 ± 0.1604

56.5368 ± 0.3665

0.1697 ± 0.0023^Abc

6.0705 ± 0.1814

3.6158 ± 0.0830

24.3598 ± 0.2842^a

68.2035 ± 0.2194

30.1933 ± 0.1991

2.2977 ± 0.0289

27.0443 ± 0.1860^b

H3H3(61–72)

0.2002 ± 0.0043

13.8767 ± 0.1663

19.1521 ± 0.2201

57.8332 ± 0.4902

0.1669 ± 0.0031^Bbc

6.3488 ± 0.2396

3.8032 ± 0.1081

25.6261 ± 0.3649^b

67.7284 ± 0.2962

30.6459 ± 0.2693

2.2496 ± 0.0391

27.5612 ± 0.2436

P

0.0513

0.0051**

0.214

0.0649

<.0001**

0.3560

0.2434

0.0053**

0.5462

0.1757

0.7634

0.0245*

Block 2

H1H2(117–139)

0.1873 ± 0.0034^A

14.1418 ± 0.1267

19.0344 ± 0.1692

57.4628 ± 0.3893

0.1705 ± 0.0024

6.1665 ± 0.1940

3.6796 ± 0.0878

24.8359 ± 0.2983^a

67.9941 ± 0.2332

30.3523 ± 0.2134

2.2848 ± 0.0307

27.5466 ± 0.1948

H1H3(59–62)

0.1908 ± 0.0043

14.0962 ± 0.1695

19.0365 ± 0.2254

57.1552 ± 0.5004

0.1698 ± 0.0030

6.6035 ± 0.2487

3.7758 ± 0.1121

25.4165 ± 0.3779

67.9988 ± 0.3013

30.2962 ± 0.2735

2.2858 ± 0.0404

27.5735 ± 0.2497

H1H4(44–55)

0.1991 ± 0.0046

13.7531 ± 0.1790

19.3446 ± 0.2403

58.2375 ± 0.5405

0.1771 ± 0.0035^A

6.2159 ± 0.2621

3.9414 ± 0.1180

25.5598 ± 0.4005

67.3613 ± 0.3257

30.8744 ± 0.2977

2.2085 ± 0.0432

27.8206 ± 0.2639

H2H2(128–149)

0.1973 ± 0.0034

14.1252 ± 0.1253

19.4270 ± 0.166^a

57.7791 ± 0.3817

0.1679 ± 0.0024

6.2908 ± 0.1912

3.6936 ± 0.0856

25.0985 ± 0.2912

67.4990 ± 0.2285

30.7484 ± 0.2092^a

2.2254 ± 0.0299

27.8762 ± 0.1924^A

H2H3(80–102)

0.1877 ± 0.0037^A

14.0980 ± 0.1432

18.8617 ± 0.1897

56.6882 ± 0.4257

0.1714 ± 0.0028

6.3426 ± 0.2113

3.6030 ± 0.0958

24.7605 ± 0.3257^a

68.2658 ± 0.2556

29.8952 ± 0.2352^b

2.3174 ± 0.0344

27.0508 ± 0.2145^B

H2H4(88–107)

0.1877 ± 0.0038^a

14.3357 ± 0.1411

18.7635 ± 0.1887^b

56.6694 ± 0.4235

0.1622 ± 0.0028^B

6.0775 ± 0.2131

3.6211 ± 0.0957

24.6392 ± 0.3234^a

68.1996 ± 0.2544

30.0572 ± 0.2341

2.3011 ± 0.0339

27.0983 ± 0.2132^Ca

H2H5(50–69)

0.2051 ± 0.0042^Bb

14.3662 ± 0.1625

19.5612 ± 0.2164^a

57.5823 ± 0.4896

0.1644 ± 0.0033

6.1397 ± 0.2402

3.8763 ± 0.1080

25.9925 ± 0.368^b

67.4171 ± 0.2947

30.8806 ± 0.2665^a

2.2230 ± 0.0391

28.0083 ± 0.2341^ACb

P

<.0001**

0.0798

0.0036**

0.0345*

0.0020**

0.5789

0.0498*

0.0075**

0.0096**

0.0015**

0.0574

0.0001**