Table 2 The Z-scores, fold increases of pBD3 expression level in parental IPEC-J2 cells of 21 hits at indicated concentrations from primary and secondary screening of the natural product library

Deoxyshikonin

6.79

20.75

3.84

43043-74-9

Quinones

Lithosperraum erythrorhizon

Scutellarein

4.73

0.68

3.30

1.29

529-53-3

Flavonoids

Scutellaria baicalensis

Pinostilbene

4.57

1.06

1.23

4.16

42438-89-1

Phenols

Pinaceae

Baicalin

4.16

1.13

3.14

2.21

21967-41-9

Flavonoids

Scutellaria baicalensis

Pterostilbene

4.00

1.33

1.57

4.38

537-42-8

Phenols

Cyanococcus

Tectorigenin

3.97

1.78

1.50

4.73

548-77-6

Flavonoids

Belamcanda chinensis (L.) DC.

Sophoricoside

3.88

1.02

2.73

1.76

152-95-4

Flavonoids

Styphnolobium japonicum

Piceatannol 3′-O-glucoside

3.74

1.41

1.90

3.03

94356-26-0

Phenols

Rheum

Isorhapontigenin

3.69

1.88

3.30

12.62

32507-66-7

Phenols

Gnetum cleistostachynm

6-Hydroxyflavone

3.55

1.36

2.91

5.90

6665-83-4

Flavonoids

Animal

Nocodazole

3.50

2.49

3.48

6.28

31430-18-9

Alkaloids

Plant

Irigenin

3.16

1.34

2.12

4.15

548-76-5

Flavonoids

Belamcanda chinensis (L.) DC.

Calycosin-7-O-beta-D-glucoside

2.90

1.32

1.23

1.28

20633-67-4

Flavonoids

Astragalus membranaceus

Dihydroresveratrol

2.84

0.79

1.66

1.13

58436-28-5

Phenols

Veratrum nigrum

Obtusifolin

2.74

1.33

1.77

4.86

477-85-0

Quinones

Cassia angustifolia

Nepetin-7-glucoside

2.61

0.96

1.11

1.76

569-90-4

Flavonoids

Salvia plebeia R.Br.

Calycosin

2.53

1.26

3.64

10.31

20575-57-9

Flavonoids

Astragalus membranaceus

Tectoridin

2.44

1.39

1.67

1.26

611-40-5

Flavonoids

Belamcanda chinensis (L.) DC.

Rhapontigenin

2.09

0.99

0.99

3.91

500-65-2

Phenols

Rheum

Xanthohumol

2.01

1.75

6.65

16.26

6754-58-1

Flavonoids

Plant

Polydatin

2.01

0.77

1.64

2.43

27208-80-6

Phenols

Fallopia japonica