Discovery of natural products capable of inducing porcine host defense peptide gene expression using cell-based high throughput screening

Table 1 Primers used in this study

Gene	Forward primer (5'→3')	Product size, bp	Accession number
GAPDH	Forward: GCTACACTGAGGACCAGGTTG Reverse: CCTGTTGCTGTAGCCAAATTC	146	XM_021091114.1
pBD2	Forward: TGTCTGCCTCCTCTCTTCC Reverse: AACAGGTCCCTTCAATCCTG	149	NM_214442.2
pBD3	Forward: CCTTCTCTTTGCCTTGCTCTT Reverse: GCCACTCACAGAACAGCTACC	163	XM_021074698.1
pEP2C	Forward: ACTGCTTGTTCTCCAGAGCC Reverse: TGGCACAGATGACAAAGCCT	92	XM_003362076.4
PG1–5	Forward: ACGGTGAAGGAGACTGTG Reverse: CGCAGAACCTACGCCTACAA	196	XM_021070622.1
IL1β	Forward: GCCCTGTACCCCAACTGGTA Reverse: CCAGGAAGACGGGCTTTTG	61	NM_001302388.2
IL8	Forward: TTCGATGCCAGTGCATAAATA Reverse: CTGTACAACCTTCTGCACCCA	176	NM_213867.1
TNFα	Forward: CCCCTCTGAAAAAGACACCA Reverse: TCGAAGTGCAGTAGGCAGAA	180	NM_214022.1

ISSN: 2049-1891