Skip to main content
Fig. 3 | Journal of Animal Science and Biotechnology

Fig. 3

From: Discovery of natural products capable of inducing porcine host defense peptide gene expression using cell-based high throughput screening

Fig. 3

Induction of multiple porcine HDP expression in IPEC-J2 cells by xanthohumol, isorhapontigenin, deoxyshikonin, and calycosin in a dose (a) and time (b)-dependent manner. Chemical structure of each compound was also shown in panel a. IPEC-J2 cells treated with xanthohumol, Isorhapontigenin, or calycosin at 5 to 320 μmol/L, or with deoxyshikonin at 0.3125 to 20 μmol/L for 24 h, followed by RT-qPCR analysis of porcine HDP genes (a). IPEC-J2 cells treated with 40 μmol/L xanthohumol, 80 μmol/L isorhapontigenin, 5 μmol/L deoxyshikonin, and 80 μmol/L calycosin for 6, 12, 24, 48 h, followed by RT-qPCR analysis of porcine HDP genes (b). The results are the mean ± SEM of three independent experiments. *P < 0.05 vs. non-treated control by unpaired Student’s t-test

Back to article page