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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Discovery of natural products capable of inducing porcine host defense peptide gene expression using cell-based high throughput screening

Fig. 1

High throughput screening of natural compounds to induce pBD3 gene expression. a, Z-scores of 1261 natural compounds following a primary screening. Stable IPEC-J2/pBD3-luc luciferase reporter cells were stimulated with natural compounds at 20 μmol/L in 96-well plates for 24 h. Four hours prior to the luciferase assay, cell viability was measured using alamarBlue cell viability assay reagent. The luciferase activity was measured using a Steady-Glo Luciferase Assay System and was normalized to cell viability before the Z-score was calculated. b, Secondary screening based on luciferase activity. IPEC-J2/pBD3-luc cells were stimulated with natural compounds at 5, 20, or 80 μmol/L in 96-well plates for 24 h. The fold change in the luciferase activity induced by each compound relative to that in non-stimulated control cells was calculated

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