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Table 1 Basic test diet formulated to allow for 13 treatment combinationsa varying in valine, isoleucine, and leucine for the response surface experiments in 22 to 35 days old male and female broilers

From: Dietary branched-chain amino acid assessment in broilers from 22 to 35 days of age

Items Inclusion, %
Corn 63.90
Peanut meal 22.00
Soybean meal 3.74
Palm olein 2.30
Palm olein fillera 2.00
Limestone 1.62
Mono-calcium phosphate 0.68
L-Lysine HCl 0.84
DL-Methionine 0.43
L-Threonine 0.34
L-Valine 0.34
L-Isoleucine 0.33
L-Tryptophan 0.07
Sodium bicarbonate 0.40
Vitamin and phytase premixb 0.25
Sodium chloride 0.18
Potassium carbonate 0.16
Mycotoxin binder 0.15
Choline chloride 0.13
Washed sand fillera 0.08
Mineral premixc 0.06
Total 100.0
Calculated nutrients, % unless otherwise noted
 Metabolizable energy, kcal/kg 3100
 Lysine, digestible % 1.13
 Methionine + cysteine, digestible % 0.85
 Threonine, digestible % 0.76
 Tryptophan, digestible % 0.20
 Arginine, digestible % 1.25
 Valine, digestible % 0.74
 Isoleucine, digestible % 0.66
 Leucine, digestible % 1.24
 Calcium, total % 0.80
 Non-phytate phosphorus, total % 0.23
 Sodium, total % 0.18
  1. aThe 13 experimental treatments were created by varying levels of L-valine, L-isoleucine, and L-leucine to achieve BCAA digestible ratios to lysine (Table 2). Palm olein and washed sand fillers were provided in varying amounts to assure all 13 treatment diets were equal in space and energy. The test diet was formulated to the lowest level of leucine from intact ingredients, and the highest levels of valine and isoleucine using a mixture of intact ingredients and feed grade amino acids
  2. bVitamin premix contained per kg of diet: vitamin A, 9030 IU; vitamin D3, 2580 IU; vitamin E, 21.50 IU; vitamin K3, 3.01; cobalamin, 0.017 mg; riboflavin, 7.74 mg; D-Pantothenic Acid, 12.04 mg; niacin, 38.7 mg; folic acid, 1.08 mg; pyridoxine, 3.01 mg; thiamine, 2.15 mg; biotin, 0.052 mg; and phytase provided 750 FTU/kg diet
  3. cMineral premix provided per kg of diet: Mn, 65 mg; Zn, 58 mg; Co, 0.14 mg; Cu, 4 mg; I, 0.22 mg; Se, 0.11 mg