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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: Lactobacillus reuteri-derived extracellular vesicles maintain intestinal immune homeostasis against lipopolysaccharide-induced inflammatory responses in broilers

Fig. 6

Treatment of macrophages with LrEVs induces anti-inflammatory responses in splenic lymphocytes. a Experimental schedule of Mφ-splenic lymphocytes coculture in vitro using a Transwell system. HD11 cells (5 × 105 cells/mL) were pretreated with PBS or LrEVs (10 μg/mL) for 12 h, washed and added to the basolateral compartment of a 6-well plate. Splenic lymphocytes (5 × 105 cells/mL) from LPS-challenged chickens were seeded on the apical compartment of 6-well Hanging Inserts and incubated with or without PBS- or LrEVs-pretreated HD11 cells for 12 h. b LrEVs increased the expression of anti-inflammatory cytokine genes IL-10 and TGF-β in splenic lymphocytes of LPS-challenged chickens during in vitro coculture with HD11 cells. c LrEVs suppressed the expression of pro-inflammatory cytokine genes IFN-γ and IL-17 in splenic lymphocytes of LPS-challenged chickens during in vitro coculture with HD11 cells. d Treatment of HD11 cells with LrEVs improved the gene expression of CD25, T-lymphocyte antigen 4 (CTLA-4) and lymphocyte activation gene 3 (LAG-3). Data are representative of three independent experiments and expressed as means ± SEM. ***P < 0.001; NS, not significant

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