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Table 4 Effects of brewers’ spent grain (BSG) protein hydrolysates or antibiotics on gas and dissolved gas production in RUSITEC

From: Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Item Treatmentsd SEM P <
Con AlcH FlaH Ant
Dissolved gase
 Total dGas, L/d 0.95 0.96 0.96 0.94 0.02 0.17
 dCH4, % of dGas 0.72a 0.68a 0.63b 0.53c 0.04 0.01
 dCH4, mg/d 4.42a 4.21a 3.86b 3.17c 0.23 0.01
 dCH4, mg/g DM digested 0.59a 0.58a 0.53b 0.45c 0.03 0.01
 dH2, μg/d 0.48 0.63 0.58 0.54 0.20 0.38
 dH2, μg/g DM digested 0.06 0.08 0.08 0.07 0.01 0.39
Gas Productionf
 Total gas, L/d 1.68 1.62 1.59 1.58 0.20 0.87
 CH4, % of gas 2.47a 2.55a 2.11b 1.32c 0.12 0.01
 CH4, mg/d 27.49a 27.15a 24.37a 14.92b 5.38 0.01
 CH4, mg/g DM digested 3.80a 3.77a 3.49a 2.19b 0.72 0.01
 H2, μg/d 105.1a 65.3b 54.3b 18.0c 11.01 0.01
 H2, μg/g DM digested 14.6a 9.0b 7.6b 2.6c 1.58 0.01
  1. a, b, c Least square means within a row with different superscripts differ (P < 0.05);
  2. d Con Control, no antioxidant peptide and no antibiotics, AlcH Alcalase hydrolysates, 10 mg per gram of TMR (DM basis), FlaH Flavourzyme hydrolysates, 10 mg per gram of TMR (DM basis), Ant antibiotics, 0.8 mg monensin + 1 mg tylosin per gram of TMR (DM basis)
  3. e Sampled from d 14 to 15
  4. f Sampled from d 9 to 13