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Table 4 Effects of brewers’ spent grain (BSG) protein hydrolysates or antibiotics on gas and dissolved gas production in RUSITEC

From: Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Item

Treatmentsd

SEM

P <

Con

AlcH

FlaH

Ant

Dissolved gase

 Total dGas, L/d

0.95

0.96

0.96

0.94

0.02

0.17

 dCH4, % of dGas

0.72a

0.68a

0.63b

0.53c

0.04

0.01

 dCH4, mg/d

4.42a

4.21a

3.86b

3.17c

0.23

0.01

 dCH4, mg/g DM digested

0.59a

0.58a

0.53b

0.45c

0.03

0.01

 dH2, μg/d

0.48

0.63

0.58

0.54

0.20

0.38

 dH2, μg/g DM digested

0.06

0.08

0.08

0.07

0.01

0.39

Gas Productionf

 Total gas, L/d

1.68

1.62

1.59

1.58

0.20

0.87

 CH4, % of gas

2.47a

2.55a

2.11b

1.32c

0.12

0.01

 CH4, mg/d

27.49a

27.15a

24.37a

14.92b

5.38

0.01

 CH4, mg/g DM digested

3.80a

3.77a

3.49a

2.19b

0.72

0.01

 H2, μg/d

105.1a

65.3b

54.3b

18.0c

11.01

0.01

 H2, μg/g DM digested

14.6a

9.0b

7.6b

2.6c

1.58

0.01

  1. a, b, c Least square means within a row with different superscripts differ (P < 0.05);
  2. d Con Control, no antioxidant peptide and no antibiotics, AlcH Alcalase hydrolysates, 10 mg per gram of TMR (DM basis), FlaH Flavourzyme hydrolysates, 10 mg per gram of TMR (DM basis), Ant antibiotics, 0.8 mg monensin + 1 mg tylosin per gram of TMR (DM basis)
  3. e Sampled from d 14 to 15
  4. f Sampled from d 9 to 13