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Table 3 Effect of brewers’ spent grain (BSG) protein hydrolysates or antibiotics on nutrient disappearances and fermentation characteristics in RUSITEC

From: Effects of brewers’ spent grain protein hydrolysates on gas production, ruminal fermentation characteristics, microbial protein synthesis and microbial community in an artificial rumen fed a high grain diet

Item

Treatmentsd

SEM

P <

Con

AlcH

FlaH

Ant

Nutrient disappearance, %

 DM

76.5a

76.1ab

75.3b

72.7c

0.58

0.01

 OM

77.7a

77.6a

76.5b

74.1c

0.56

0.01

 CP

75.1a

75.4a

73.4b

69.7c

0.63

0.01

 NDF

40.3a

38.7a

38.9a

31.8b

1.86

0.01

 ADF

29.2a

26.8a

27.2a

21.5b

1.88

0.01

 Starch

90.7a

90.8a

89.5b

87.8c

0.50

0.01

Fermentation characteristics

 pH

5.74b

5.78b

5.77b

5.85a

0.02

0.01

 NH3-N, mmol/d

3.66b

4.05a

4.19a

2.57c

0.12

0.01

 Total VFA, mmol/d

54.80a

54.51a

53.63a

48.88b

0.86

0.01

Individual VFA, % of total VFA

 Acetate (A)

30.97a

31.12a

31.67a

29.77b

0.38

0.03

 Propionate (P)

39.33b

39.39b

39.42b

42.98a

0.39

0.01

 Butyrate

20.37a

19.98a

19.20b

16.76c

0.45

0.01

 BCVFAe

2.21a

2.19a

2.19a

1.56b

0.03

0.01

 Valerate

4.25b

4.33b

4.78b

7.12a

0.41

0.01

 Caproate

2.64a

2.77a

2.80a

1.57b

0.17

0.01

 A:P

0.79a

0.79a

0.80a

0.69b

0.01

0.01

  1. a, b, c Least square means within a row with different superscripts differ (P < 0.05)
  2. d Con Control, no antioxidant peptide and no antibiotics, AlcH Alcalase hydrolysates, 10 mg per gram of TMR (DM basis), FlaH Flavourzyme hydrolysates, 10 mg per gram of TMR (DM basis), Ant antibiotics, 0.8 mg monensin + 1 mg tylosin per gram of TMR (DM basis)
  3. e Branched-chain volatile fatty acids (isobutyrate + isovalerate)