Fig. 3
From: In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5 °C
Sublethal damage of sperm function under capacitating and non-capacitating conditions in semen samples preserved in AndroStar® Premium extender and stored at 17 °C with antibiotics (0.25 g/L gentamicin sulphate) or at 5 °C without antibiotics (n = 9 boars, experiment 1): Distribution of viable (Hoechst 33258 negative) spermatozoa (%) with high (JC-1 aggregate positive) or low (JC-1 aggregate negative) mitochondria membrane potential and high (Calbryte 630 positive) or low (Calbryte 630 negative) intracellular calcium a after incubation in non-capacitating medium (Tyrode C) and b after incubation in capacitating medium (Tyrode A). *Values of same sperm classes differ between storage temperature groups at a given time point (P < 0.05). #Values for overall sperm distribution in all four classes differ between storage temperature groups at a given time point (P < 0.05)