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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: MicroRNA bta-miR-365-3p inhibits proliferation but promotes differentiation of primary bovine myoblasts by targeting the activin A receptor type I

Fig. 6

Knock-down of ACVR1 inhibited primary myoblast proliferation but promoted PBMs differentiation. a RNA interference using siACVR1-1 decreases expression of ACVR1 at mRNA and protein levels compared with the control group by qRT-PCR and western blotting in PBMs, respectively. b The mRNA and protein expression levels of CDK2, CCND1, and PCNA at 24 h. post transfection with siACVR1-1 in PBMs. c EdU proliferation assay detected the cell proliferation index. EdU (red), Hoechst (blue), scale bars 2000 μm. d Statistics of the percentage of dual positive cells. Error bars indicates the SD among three repeats. e The mRNA and protein expression of MYOG in PBMs after treated with siACVR1-1 and induced differentiation for four days. f The mRNA and protein expression of MYOD1 in PBMs after treated with siACVR1-1 induced for four days. Values are mean ± SD. Note: CDK2: cyclin-dependent kinase 2; PCNA: Proliferating cell nuclear antigen; MYOD1: Myogenic differentiation 1; MYOG: Myogenin; siACVR1: The interference RNA of ACVR1 in cattle; SD: standard deviation. *P < 0.05; *** P < 0.001

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Journal of Animal Science and Biotechnology

ISSN: 2049-1891