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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: MiR-146a-5p targeting SMAD4 and TRAF6 inhibits adipogenensis through TGF-β and AKT/mTORC1 signal pathways in porcine intramuscular preadipocytes

Fig. 6

MiR-146a-5p inhibitor accelerates porcine intramuscular preadipocyte differentiation. a Porcine intramuscular preadipocyte were treated with miR-146a-5p inhibitor to induce differentiation on the 6th day. a, b Bodipy or AdipoRed staining was performed on lipid droplets. c White light field and oil red O stained lipid droplets. d After extracting oil red O with isopropanol, the OD value (510 nm) was detected. e Interference efficiency of miR-146a-5p inhibitor after transfection for 6d. f RT-qPCR was used to detect the adipogenesis genes C/EBPβ, PPARγ, and FABP4. g Western blot analysis of C/EBPβ, PPARγ, and FABP4 after transfection with miR-146a-5p inhibitor. h Protein quantitative analysis of g. Values are expressed as the mean ± SE (n = 3). *, P < 0.05; **, P < 0.01, versus INC

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