Fig. 2

MiR-146a-5p mimics inhibit proliferation of porcine intramuscular preadipocytes. a EdU staining assay. Porcine intramuscular preadipocyte in the S-phase were stained with EdU in red, and cell nuclei were dyed with Hoechst in blue. b Quantification ratio of EdU-positive cells/total cells. c CCK-8 analysis after treatment with miR-146a-5p mimics during porcine intramuscular preadipocyte proliferation. d Cell cycle analysis of preadipocyte by flow cytometry. e Statistical results of flow cytometry. f Overexpression efficiency of miR-146a-5p mimics after transfection for 24 h. g RT-qPCR was used to detect the cell cycle genes cyclin B, cyclin D, cyclin E, and p21. h Western blot analysis of cyclin E, cyclin D, PCNA and p21 after transfection with miR-146a-5p mimics. i Protein quantitative analysis of cyclin E, cyclin D, PCNA and p21. Values are expressed as mean ± SEM (n = 3). *, P < 0.05; **, P < 0.01, versus MNC