Fig. 3

Differential expression levels of mRNAs and lncRNAs in adipogenic (Adi-) and myogenic (Myo-) precursors and validation of RNAseq results by qRT-PCR analysis. a Volcano map of all mRNAs and lncRNAs between Adi and Myo. b The up- and down-regulated number of mRNA and lncRNA between Adi and Myo. 572 up-regulated mRNAs, 183 down-regulated mRNAs, 9 up-regulated lncRNAs, and 15 down-regulated lncRNAs in myogenic precursors. Differentially expressed mRNAs (c) and lncRNAs (d) were clustered on the heat map by the normalized expression abundance. Relative lncRNA (e) and mRNA (f) expression were normalized to GAPDH. n = 4. Data are presented as mean ± SEM. g The correlation of fold change of differential expressed lncRNAs and mRNAs expression between the RNA-seq and RT-qPCR. A paired two-tailed Student’s t-test was used. * P < 0.05, ** P < 0.01