Fig. 1

Characterization of adipogenic (Adi-) and myogenic (Myo-) precursors derived from the skeletal muscle of neonatal pigs (n = 3). a Oil Red O staining and immunofluorescence of adipogenic and myogenic precursors following 9-d adipogenic induction and 3-d myogenic induction, respectively. Oil Red O (red), Myosin (red), and DAPI (blue). b OD of Oil Red O at 520 nm and differentiation index between Adi and Myo. c Differentiation index between Adi and Myo. d Quantitative RT-PCR for the mRNA expression level of adipogenesis specific genes (PPARγ, C/EBPα, and LPL) and myogenesis specific genes (MYF5, MYOD1, and MYOG) following adipogenic or myogenic induction. Data are presented as mean ± SEM. Student’s t-test was used. Adi, adipogenic precursors; Myo, myogenic precursors. Scale bars, 100 μm. *P < 0.05, **P < 0.01 compared with adipogenic precursors