Skip to main content
Fig. 7 | Journal of Animal Science and Biotechnology

Fig. 7

From: Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids

Fig. 7

Time-restricted feeding modifies histones modification on the loci of RORγ binding. a Putative co-factors of RORγ transcriptional regulation were predicted by STRING. b-d The relative enrichment of RORγ co-factors (p300, SRC-1 and SRC-3) at the locus of MVK in organoids analyzed by ChIP-qPCR. e-i The relative enrichment of histone marks (H3K27ac, H3K4me1/2/3) and RNA polymerase II occupancy analyzed by ChIP-qPCR. j-l RORγ agonists SR0987 and Desmosterol enhanced the occupancies of p300, H3K27ac and H3K4me1 at target loci MVK of TRF treated organoids. Data were presented as means ± SD of at least three independent experiments, *P < 0.05, **P < 0.01. using ANOVA with Tukey’s post hoc test

Back to article page

Journal of Animal Science and Biotechnology

ISSN: 2049-1891