Fig. 2

Time-restriction feeding does not affect cell survival and growth in porcine liver organoids. a In the established porcine liver organoids model, representative images show the cell viability determined using live/dead reagent staining. b The percentage of viable cells (%) quantified by measuring ATP presence, an indicator of metabolically active cells. c The cell apoptosis in the porcine liver organoids reflected by the relative caspase 3/7 enzyme activity. d The relative mRNA expression of key genes involved in cell cycle/proliferation. Data were presented as means ± SD of at least three independent experiments, *P < 0.05, using two tailed Student's t-test-