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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Time-restricted feeding downregulates cholesterol biosynthesis program via RORγ-mediated chromatin modification in porcine liver organoids

Fig. 1

Illustration of the experimental design. The porcine liver organoids are derived from biopsies of normal liver tissues that can be used as a model in basic research and drug testing. The organoids were placed in Matrigel with the optimized media for 14 days after seeding and were treated with dexamethasone (DEX) for synchronization. They were subjected to the 24-h cycle of time-restricted feeding for 7 consecutive days: for the control group, the organoids were exposed to expansion medium for 14 h from 8:00 to 22:00 and to basal medium for 10 h from 22:00 to 8:00(+1 d); For the TRF group, the exposure to expansion medium was restricted to 10 h from 8:00 to 18:00 and to basal medium for 14 h from 18:00 to 8:00 (+1 d)

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