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Table 1 Composition and nutritional content of the experimental diets

From: Modulations of genes related to gut integrity, apoptosis, and immunity underlie the beneficial effects of Bacillus amyloliquefaciens CECT 5940 in broilers fed diets with different protein levels in a necrotic enteritis challenge model

Ingredients, % Starter (d 0–10) Grower (d 11–16)
SCPc RCPd SCP RCP
Wheat 39.9 49.5 42.6 52.3
Soybean meal 33.2 24.1 29.2 20.0
Sorghum 20.0 20.0 20.0 20.0
Canola oil 2.83 1.38 3.93 2.47
Dicalcium phosphate 1.27 1.33 1.09 1.15
Limestone 1.24 1.26 1.16 1.18
Salt 0.55 0.55 0.55 0.56
DL-Methionine 0.34 0.38 0.31 0.35
L-Lysine HCl 78% 0.27 0.52 0.25 0.50
L-Threonine 0.16 0.27 0.14 0.25
Vitamin premixa 0.08 0.08 0.08 0.08
Trace mineral premixb 0.10 0.10 0.10 0.10
L-Valine 0.12 0.12
L-Isoleucine 0.11 0.11
L-Arginine 0.18 0.19
Choline chloride 60% 0.03 0.07 0.03 0.07
Phytase 0.01 0.01 0.01 0.01
Titanium dioxide 0.50 0.50
Nutrients
 CP,% 23.00 21.00 21.5 19.5
 AMEn, kcal/kg 3000 3000 3090 3090
 Dig. Lys, % 1.20 1.20 1.10 1.10
 Dig. Met, % 0.62 0.62 0.57 0.57
 Dig. Arg, % 1.33 1.33 1.22 1.22
 Dig. Ile, % 0.83 0.83 0.77 0.77
 Dig. Val, % 0.90 0.90 0.84 0.84
 Choline, mkg/kg 1700 1700 1600 1600
 Linoleic acid, % 1.68 1.25 1.99 1.56
 Ca, % 0.96 0.96 0.87 0.87
 Av. P, % 0.48 0.48 0.44 0.44
  1. aSupplied per kilogram of diet: vitamin A, 8255 IU; vitamin D3, 3000, IU; vitamin E, 30 IU; vitamin K, 2 mg; thiamine (vitamin B1), 4 mg; riboflavin (vitamin B2), 6 mg; niacin, 41.2 mg; folic acid, 1 mg; biotin, 0.25 mg; pyridoxine, 4 mg; choline, 1301 mg; pantothenic acid, 11 mg; vitamin B12, 0.013 mg. bSupplied per kilogram of diet: Cu (sulphate), 16 mg; Fe (sulphate), 40 mg; I (iodide), 1.25 mg; Se (selenate), 0.3 mg; Mn (sulphate and oxide), 120 mg; Zn (sulphate and oxide), 100 mg; cereal-based carrier, 128 mg; mineral oil, 3.75 mg., cStandard crude protein. dReduced crude protein