Dietary L-citrulline supplementation modulates nitric oxide synthesis and anti-oxidant status of laying hens during summer season

Table 1 Calculated dietary composition and nutrient levels of experimental diets

	Control	0.25% L-Cit	0.50% L-Cit	1.00% L-Cit
Corn (8.5% CP)	54.055	53.805	53.555	53.055
Wheat bran	7.99	7.99	7.99	7.99
Soya bean oil	1.79	1.79	1.79	1.79
Soybean meal (43%)	24.29	24.29	24.29	24.29
Salt	0.35	0.35	0.35	0.35
Limestone	9.6	9.6	9.6	9.6
Dicalcium phosphate	1.44	1.44	1.44	1.44
Choline chloride (50%)	0.09	0.09	0.09	0.09
L- Lysine (99%)	0.1	0.1	0.1	0.1
DL- Methionine (99%)	0.17	0.17	0.17	0.17
Mineral premix ^a (0.1%)	0.1	0.1	0.1	0.1
Vitamin premix ^b (0.025%)	0.025	0.025	0.025	0.025
L-citrulline (98%)	0	0.25	0.5	1
Total	100	100	100	100
Nutrients levels
Total CP, %	16.5	16.85	17.19	17.89
Non-citrulline CP, %	16.5	16.48	16.46	16.42
Metabolizable energy, kcal/kg	2700	2700	2700	2700
Calcium, %	3.5	3.5	3.5	3.5
Available phosphorus, %	0.404	0.404	0.404	0.404
Digestible lysine, %	0.78	0.78	0.78	0.78
Digestible methionine, %	0.4	0.4	0.4	0.4
Digestible methionine + cysteine, %	0.619	0.619	0.618	0.616
Digestible threonine, %	0.505	0.504	0.504	0.502
Digestible tryptophan, %	0.161	0.161	0.161	0.161

^a The mineral premix provide the follow quantities per kilogram of diet: iron, 55 mg; selenium, 0.3 mg; copper, 5.5 mg; zinc, 88 mg; I, 1.7 mg; manganese, 88 mg.
^b The vitamin premix provide the follow quantities per kilogram of diet: vitamin A, 8800 IU; vitamin D₃, 3300 IU; vitamin K, 2.2 mg; vitamin E, 16.5 IU; cholecalciferol, 2800 IU; riboflavin 18.0 mg; niacin, 50 mg; pantothenic acid, 28 mg; biotin, 0.1 mg; folic acid, 0.6 mg

ISSN: 2049-1891