Fig. 9
From: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1
MiR-101-3p inhibits PI3K-AKT-mTOR pathway via STC1. Goat granulosa cells are transfected (a) miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in), (b) siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control), (c) miR-101-3p-mi or NC-mi or co-transfected miR-101–3p-mi with pcDNA3.1-STC1 for 48 h. Cytosolic protein and related phosphorylation levels for PI3K, AKT, mTOR and PTEN are analysed using Western blot. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance