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Fig. 8 | Journal of Animal Science and Biotechnology

Fig. 8

From: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1

Fig. 8

MiR-101-3p regulates the apoptotic genes in granulosa cells via STC1. Goat granulosa cells are transfected with miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in). a The mRNA expressions of apoptotic genes (Bcl-2, Bax and p53) are detected using RT-qPCR after 24 h transfection. b The protein expressions of apoptotic genes (Caspase 3, Bcl-2, Bax and p53) are detected using Western blot after 48 h transfection. Caspase 3, Bcl-2, Bax and p53 protein levels are detected in granulosa cells transfected with (d) siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control), (e) miR-101-3p-mi or NC-mi or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 for 48 h using Western blot. (c, f) Histograms show the ratio of Bcl-2/Bax. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance

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