Fig. 7
From: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1
MiR-101-3p promotes granulosa cell apoptosis via STC1. Goat granulosa cells are transfected with (a) miR-101-3p mimics (miR-101-3p-mi) or mimics NC (NC-mi), miR-101-3p inhibitors (miR-101-3p-in) or inhibitors NC (NC-in), (b) siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control), (c) miR-101-3p-mi or NC-mi or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 for 24 h. The apoptotic rates of granulosa cells are detected by flow cytometry. Histograms show the apoptotic cell distribution and percentage. Q1 means death cells, Q2 means late-state apoptotic cells, Q3 means early-state apoptotic cells and Q4 means viable non-apoptotic cells. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance