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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Effects of miR-101-3p on goat granulosa cells in vitro and ovarian development in vivo via STC1

Fig. 4

MiR-101-3p promotes steroid hormone synthesis via STC1. ELISA kits measure oestrogen (E2) and progesterone (P4) secretions in 50 μL cell-free supernatants in goat granulosa cells transfected with (a) siRNA-STC1 (si-STC1) or NC, pcDNA3.1-STC1 or pcDNA3.1 (control) or (b) miR-101-3p mimics (miR-101-3p-mi), mimics NC (NC-mi) or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 for 24 h. The protein expressions of steroid hormone synthesis-associated vital genes STAR, CYP11A1, CYP19A1 and 3β-HSD are quantified using Western blot in goat granulosa cells transfected with (c) si-STC1 or NC, pcDNA3.1-STC1 or pcDNA3.1 (control) or (d) miR-101-3p-mi, NC-mi or co-transfected miR-101-3p-mi with pcDNA3.1-STC1 after 48 h transfection. β-actin is used as an internal control. Values are expressed as mean ± SD of n = 3. * P < 0.05, ** P < 0.01. Superscripts (a, b, c) show significant difference. NS means no significance

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