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Fig. 6 | Journal of Animal Science and Biotechnology

Fig. 6

From: MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells

Fig. 6

Inhibitor of miR-214-3p promotes porcine GC estradiol synthesis. MiR-214-3p agomir or NC were transfected into cells which were harvested after 24 h. a Overexpression efficiency of miR-214-3p after transfection with miR-214-3p antagomir compared to NC; b Estradiol concentration detected by ELISA. Culture supernatants collected 24 h after miR-214-3p antagomir and NC treatment; c RT-qPCR detects E2 synthesis-related genes including Star, Cyp11a1, and Cyp19a1 after 24-h transfection; d Western blot analysis of E2 synthesis-related genes; e Quantification of Western blot analysis of StAR, CYP11A1, CYP19A1. Note: Data are mean ± SEM of three independent experiments; * P < 0.05, ** P < 0.01

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