Fig. 5
From: MiR-214-3p promotes proliferation and inhibits estradiol synthesis in porcine granulosa cells
Overexpression of miR-214-3p inhibits porcine GC estradiol synthesis. MiR-214-3p agomir or NC transfected into cells harvested after 24 h. a Overexpression efficiency of miR-214-3p after transfection with miR-214-3p agomir compared to NC; b Estradiol concentration detected by ELISA. Culture supernatants collected 24 h after miR-214-3p agomir and NC treatment; c RT-qPCR detects E2 synthesis-related genes including Star, Cyp11a1, and Cyp19a1 after 24-h transfection; d Western blot analysis of E2 synthesis-related genes; (E) Quantification of Western blot analysis of StAR, CYP11A1, CYP19A1. Note: Data are mean ± SEM of three independent experiments; * P < 0.05, ** P < 0.01