Fig. 4

MiR-214-3p targets Mfn2 during GC proliferation. a miR-214-3p binding site within the Mfn2 3′-UTR predicted by RNAhybrid; b Target site of miR-214-3p within porcine Mfn2 mRNA 3’UTR and mutational site of Mfn2 3’UTR; c Dual luciferase assay by co-transfection of miR-214-3p agomir and wild-type vectors or mutant vectors. Relative luciferase activity represented by Renilla Luciferase/Firefly Luciferase (RLUC/FLUC); d Relative Mfn2 mRNA expression levels after treatment with miR-214-3p agomir; e Relative Mfn2 mRNA expression levels after treatment with miR-214-3p antagomir; f Western blot analysis of Mfn2 protein expression after treatment with miR-214-3p agomir; h Western blot analysis of Mfn2 protein expression after treatment with miR-214-3p antagomir; g,i Mfn2 protein level quantifications. Note: Data are mean ± SEM of three independent experiments. * P < 0.05, ** P < 0.01