Fig. 1

Metabolic activity of murine macrophages after treatment with components of Saccharomyces cerevisiae. Metabolic activity was assessed by metabolism of resazurin to the fluorescent product resorufin. Untreated RAW cells represent the negative control, and RAW cells stimulated with 0.1 μg/mL LPS served as the positive control. Increasing doses (0.01, 0.1, or 1 mg/mL) are signified by increasing color intensity within treatment compound. Solid bars indicate control or cells treated with yeast components, whereas patterned bars indicate cells stimulated with LPS or both LPS and yeast components. Values are reported as LSM ± SE. * indicates means (yeast components alone) differ significantly from CON, # indicates means (yeast components following LPS) differ significantly from LPS (P < 0.05, Tukey’s HSD). Different letters indicate means differ (P < 0.05, Tukey’s HSD). Overall, yeast treatment interacted with LPS stimulation (interaction, P < 0.01) on cellular metabolism of resazurin