Fig. 1From: Identification of novel molecular markers of mastitis caused by Staphylococcus aureus using gene expression profiling in two consecutive generations of Chinese Holstein dairy cattleIdentification of S. aureus isolated from milk samples from Holstein cows. a Workflow of sample selection. A total of 38 mother-daughter pairs were selected from 1,200 lactating dairy cattle according to pedigree and SCC records. After S. aureus identification from milk samples, four S. aureus-positive and four S. aureus-negative pairs were used for subsequent studies. b Specific PCR and electrophoresis map of the S. aureus thermonuclease (nuc) gene (279 bp). SA: abbreviation of S. aureus. +: S. aureus-positive control; −: S. aureus-negative control. SM: S. aureus mastitis mother; HM: healthy mother; SMD: S. aureus mastitis daughter; HMD: healthy daughter. c Sequence alignment of partial nuc gene. The upper lines were amplified from samples collected from the cows’ milk; the lower line is nuc sequence amplified from one of the S. aureus-positive samples. Identity = 95.68%Back to article page