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Table 1 Primers used for BTN knockout and identification

From: Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

Primer name

Primer sequence (5′→3′)1

Primer function

BTN-sgRNA1-F

caccGTTTCCAAACTCCTGCCTCGC

Knockout exon 2 gene of bovine BTN1A1 gene

BTN-sgRNA1-R

aaacGCGAGGCAGGAGTTTGGAAAC

 

BTN-sgRNA2-F

caccGCAGCTGCCCAAGCTGGATTC

Knockout exon 2 gene of bovine BTN1A1 gene

BTN-sgRNA2-R

aaacGAATCCAGCTTGGGCAGCTGC

 

BTN-sgRNA3-F

caccGACCCCCGGAGCCCATCCTGG

Knockout exon 3 gene of bovine BTN1A1 gene

BTN-sgRNA3-R

aaacCCAGGATGGGCTCCGGGGGTC

 

BTN-Test1-F

TGCCTTCTCCTAAGACTCTCTTGG

T7 EI enzyme digestion of exon 2 gene sequence

BTN-Test1-R

GATAATTCGCAGCTCCTTCTCTA

 

BTN-Test2-F

TAGAGAAGGAGCTGCGAATTATC

T7 EI enzyme digestion of exon 3 gene sequence

BTN-Test2-R

ATCATCAGAGGCTTTGACCTCCTG

 

PPARγ–qPCR-F

CCAAATATCGGTGGGAGTCG

qPCR for PPAR γ gene

PPARγ–qPCR-R

ACAGCGAAGGGCTCACTCTC

 

ADRP–qPCR-F

CATCTGTTGCAGTTGAACCAC

qPCR for ADRP gene

ADRP–qPCR-R

AAGCCGAGGAGACCAGATCA

 

FASN–qPCR-F

ACCTCGTGAAGGCTGTGACTCA

qPCR for FASN gene

FASN–qPCR-R

TGAGTCGAGGCCAAGGTCTGAA

 
  1. 1cacc and aaac: complementary bases