Primer name | Primer sequence (5′→3′)1 | Primer function |
---|---|---|
BTN-sgRNA1-F | caccGTTTCCAAACTCCTGCCTCGC | Knockout exon 2 gene of bovine BTN1A1 gene |
BTN-sgRNA1-R | aaacGCGAGGCAGGAGTTTGGAAAC | Â |
BTN-sgRNA2-F | caccGCAGCTGCCCAAGCTGGATTC | Knockout exon 2 gene of bovine BTN1A1 gene |
BTN-sgRNA2-R | aaacGAATCCAGCTTGGGCAGCTGC | Â |
BTN-sgRNA3-F | caccGACCCCCGGAGCCCATCCTGG | Knockout exon 3 gene of bovine BTN1A1 gene |
BTN-sgRNA3-R | aaacCCAGGATGGGCTCCGGGGGTC | Â |
BTN-Test1-F | TGCCTTCTCCTAAGACTCTCTTGG | T7 EI enzyme digestion of exon 2 gene sequence |
BTN-Test1-R | GATAATTCGCAGCTCCTTCTCTA | Â |
BTN-Test2-F | TAGAGAAGGAGCTGCGAATTATC | T7 EI enzyme digestion of exon 3 gene sequence |
BTN-Test2-R | ATCATCAGAGGCTTTGACCTCCTG | Â |
PPARγ–qPCR-F | CCAAATATCGGTGGGAGTCG | qPCR for PPAR γ gene |
PPARγ–qPCR-R | ACAGCGAAGGGCTCACTCTC |  |
ADRP–qPCR-F | CATCTGTTGCAGTTGAACCAC | qPCR for ADRP gene |
ADRP–qPCR-R | AAGCCGAGGAGACCAGATCA |  |
FASN–qPCR-F | ACCTCGTGAAGGCTGTGACTCA | qPCR for FASN gene |
FASN–qPCR-R | TGAGTCGAGGCCAAGGTCTGAA |  |