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Fig. 4 | Journal of Animal Science and Biotechnology

Fig. 4

From: Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

Fig. 4

Effect of knockout of BTN1A1 on lipid droplet (LD) formation in bovine mammary epithelial cells (BMEC). All experiments were conducted in triplicate. All LD visualised within cells were measured using CellSens Entry software (version 1.7; Olympus). Data are reported as Means ± SE. a Nile Red staining of LD was performed after 12, 24 and 48 h of cell culture. Relative fluorescence intensity of LD was quantified at an excitation wavelength of 480 nm and an emission wavelength of 575 nm in a full-wavelength microplate reader. b LD were stained red by Oil-Red-O, and nuclei were re-stained blue by hematoxylin. c The average LD diameter (μm) was determined by analysis 120 randomly chosen LD per group. d Number of LD of different sizes. Lipid droplets were divided into four size groups (0 < size < 2.0 μm, 2.0 μm < size < 2.5 μm, 2.5 μm < size < 3.0 μm and size > 3.0 μm). Number of LD was counted within the four sizes. WT, wild-type; BTN1A1(−/−), BTN1A1 gene knockout cells (*P < 0.05,**P < 0.01)

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