Skip to main content
Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Knockout of butyrophilin subfamily 1 member A1 (BTN1A1) alters lipid droplet formation and phospholipid composition in bovine mammary epithelial cells

Fig. 2

Selection and identification of a BTN1A1 knockout clone. a Design of three sgRNA (sgRNA1, sgRNA2 and sgRNA3) targeting exons 2 and 3 of the BTN1A1 gene. b After puromycin selection of transfected cells, T7EI assays were performed to evaluate the genome editing effect. Lane M, 50 bp DNA markers; C−, PCR products of wild-type (WT) cells used as a negative control. c Sequences of modified BTN1A1 alleles in mutant clones. d TA clone sequencing showing a10-bp deletion the BTN1A1 gene in clone 8. The single horizontal red line indicates the deleted base pair next to the protospacer adjacent motif

Back to article page