Table 1 Milk fat globule membrane proteins secreted by 10 primiparous (PP) and 10 multiparous (MP) Holsteins at four milkings postpartum (M1, M2, M4, M14) that were affected by milking (MIL) and had lower abundance at M14 compared with M1
Accession number | Description | M1 | M2 | M4 | M14 | P-value | ||||
|---|---|---|---|---|---|---|---|---|---|---|
Value | SE | Value | SE | Value | SE | Value | SE | |||
A0A0A0MP90 | Histone H2A | 92.0 | 26.1 | 106.2 | 15.5 | 103.2 | 24.2 | 30.7 | 6.2 | <.0001 |
A0A0N4STN1 | Cathelicidin-1-like | 116.1 | 14.6 | 100.6 | 10.6 | 96.2 | 7.3 | 63.7 | 8.6 | 0.0043 |
A0A140T881 | Apolipoprotein E | 116.8 | 11.5 | 110.7 | 10.1 | 100.8 | 5.7 | 78.5 | 6.7 | 0.0079 |
A8DC37 | Fc-gamma-RII-D | 154.7 | 14.3 | 148.0 | 17.8 | 84.6 | 7.5 | 44.8 | 3.2 | <.0001 |
E1BF48 | CD177 molecule | 94.8 | 27.0 | 108.1 | 14.7 | 97.0 | 18.8 | 32.8 | 5.9 | <.0001 |
E1BGN3 | Histone H3 | 83.1 | 27.1 | 97.1 | 12.7 | 102.3 | 22.6 | 36.6 | 6.1 | <.0001 |
F1MCF8 | IGL@ protein | 146.8 | 34.6 | 86.4 | 9.8 | 56.0 | 7.4 | 21.1 | 2.7 | <.0001 |
F1MH40 | IGK protein | 151.6 | 26.9 | 99.3 | 9.2 | 64.7 | 7.8 | 29.4 | 3.1 | <.0001 |
F1MHH9 | Low affinity immunoglobulin gamma Fc region receptor II | 146.2 | 22.9 | 112.7 | 15.9 | 80.8 | 9.5 | 45.1 | 3.8 | <.0001 |
F1MHS5 | Protein S100-A9 | 115.2 | 26.6 | 109.7 | 16.2 | 99.8 | 16.3 | 32.0 | 5.3 | <.0001 |
F1MLW8 | Immunoglobulin lambda-1 light chain-like isoform X5 | 133.9 | 20.2 | 84.2 | 10.4 | 69.3 | 16.6 | 35.2 | 3.7 | <.0001 |
F1MLZ1 | Cytochrome b reductase 1 | 143.2 | 25.3 | 95.9 | 16.0 | 77.8 | 8.5 | 39.3 | 5.0 | <.0001 |
F1MMW8 | Serum amyloid A protein | 116.1 | 11.0 | 118.7 | 18.7 | 108.5 | 10.4 | 69.4 | 8.1 | 0.0016 |
F1MUD2 | Histone H2B | 88.5 | 36.8 | 102.2 | 14.1 | 104.3 | 26.5 | 32.8 | 6.7 | <.0001 |
F1MX83 | Protein S100 | 134.6 | 19.0 | 119.9 | 9.4 | 95.4 | 8.5 | 61.6 | 6.3 | <.0001 |
F1MXX6 | Lactadherin | 130.5 | 11.0 | 118.7 | 12.8 | 92.9 | 8.4 | 75.6 | 8.9 | 0.001 |
F1N1I6 | Gelsolin | 124.6 | 13.1 | 107.7 | 9.1 | 95.2 | 6.1 | 65.5 | 5.9 | <.0001 |
F1N4Y5 | Non-classical MHC class I antigen precursor | 131.4 | 10.1 | 130.4 | 15.1 | 94.8 | 7.8 | 68.4 | 5.4 | <.0001 |
F1N514 | CD5 antigen-like precursor | 153.3 | 14.0 | 115.8 | 12.5 | 62.4 | 4.8 | 32.7 | 3.1 | <.0001 |
F1N5M2 | Vitamin D-binding protein | 147.1 | 17.2 | 96.6 | 11.2 | 67.9 | 6.5 | 40.0 | 3.4 | <.0001 |
F1N650 | Annexin | 97.5 | 29.5 | 86.1 | 14.9 | 117.3 | 19.2 | 50.5 | 7.6 | 0.0044 |
F1N726 | Glycoprotein 2 | 97.9 | 12.3 | 132.2 | 13.7 | 94.6 | 11.7 | 51.1 | 7.7 | <.0001 |
G3MWV5 | Histone cluster 1 H1 family member e | 89.9 | 41.4 | 94.2 | 13.7 | 86.9 | 23.5 | 21.9 | 3.3 | <.0001 |
G3MXB5 | Immunoglobulin IgA heavy chain constant region, partial | 110.4 | 13.3 | 132.8 | 13.8 | 69.7 | 8.4 | 26.0 | 4.7 | <.0001 |
G3N0V0 | Secreted immunoglobulin gamma2 heavy chain constant region, partial | 137.1 | 37.2 | 67.9 | 10.2 | 81.0 | 26.7 | 30.1 | 5.3 | 0.0005 |
G3N2D7 | Immunoglobulin light chain, partial | 105.0 | 9.0 | 83.0 | 6.8 | 76.1 | 6.5 | 45.8 | 4.2 | <.0001 |
G3X7A5 | Complement C3 | 123.4 | 17.5 | 91.1 | 8.8 | 102.2 | 12.6 | 71.1 | 6.5 | 0.0124 |
G5E513 | IgM heavy chain constant region, secretory form, partial | 150.9 | 14.4 | 119.0 | 14.7 | 65.2 | 5.2 | 36.5 | 3.3 | <.0001 |
G5E5T5 | Immunoglobulin M heavy chain secretory form | 146.2 | 13.8 | 117.2 | 13.2 | 67.6 | 5.1 | 45.8 | 3.7 | <.0001 |
G5E5V1 | Immunoglobulin iota chain-like, partial (TPA) | 135.3 | 21.0 | 94.0 | 8.0 | 65.1 | 8.9 | 28.9 | 4.9 | <.0001 |
G5E604 | TPA: immunoglobulin iota chain-like | 136.0 | 16.6 | 95.9 | 12.3 | 66.4 | 12.1 | 34.6 | 4.1 | <.0001 |
P01888 | Beta-2-microglobulin | 110.0 | 46.5 | 93.0 | 16.6 | 90.1 | 16.4 | 35.3 | 5.6 | 0.0004 |
P08037 | Beta-1,4-galactosyltransferase 1 | 127.3 | 13.3 | 132.0 | 17.3 | 92.3 | 13.9 | 54.1 | 5.9 | <.0001 |
P08728 | Keratin, type I cytoskeletal 19 | 116.9 | 10.5 | 134.6 | 13.1 | 91.0 | 8.2 | 51.4 | 4.6 | <.0001 |
P13753 | BOLA class I histocompatibility antigen, alpha chain BL3–7 | 107.6 | 10.0 | 127.8 | 22.4 | 100.8 | 7.8 | 75.1 | 7.0 | 0.0103 |
P15497 | Apolipoprotein A-I | 135.1 | 10.2 | 141.0 | 17.1 | 116.3 | 7.5 | 73.7 | 5.4 | <.0001 |
P17697 | Clusterin | 148.6 | 11.2 | 139.7 | 13.8 | 75.3 | 7.4 | 37.1 | 3.8 | <.0001 |
P19035 | Apolipoprotein C-III | 145.0 | 15.3 | 142.5 | 18.4 | 77.4 | 5.4 | 45.6 | 3.6 | <.0001 |
P24627 | Lactotransferrin | 118.0 | 32.4 | 76.6 | 9.8 | 75.5 | 8.5 | 43.7 | 4.3 | 0.0003 |
P28782 | Protein S100-A8 | 110.9 | 23.9 | 113.4 | 16.2 | 95.5 | 17.3 | 30.9 | 4.3 | <.0001 |
P48616 | Vimentin | 112.6 | 18.7 | 108.2 | 10.5 | 104.8 | 13.4 | 51.7 | 5.5 | <.0001 |
P56425 | Cathelicidin-7 | 92.7 | 17.0 | 115.3 | 21.0 | 120.7 | 16.0 | 47.5 | 5.7 | <.0001 |
P60712 | Actin, cytoplasmic 1 | 126.6 | 19.0 | 109.4 | 10.4 | 90.4 | 7.4 | 58.8 | 5.1 | <.0001 |
P62261 | 14–3-3 protein epsilon | 121.9 | 8.6 | 116.9 | 11.2 | 99.3 | 8.1 | 87.8 | 9.4 | 0.0397 |
P62803 | Histone H4 | 98.0 | 35.8 | 105.6 | 12.3 | 108.2 | 23.7 | 36.4 | 6.4 | <.0001 |
P62992 | Ubiquitin-40S ribosomal protein S27a | 129.0 | 11.2 | 112.8 | 11.3 | 113.8 | 11.1 | 90.2 | 7.8 | 0.0338 |
P79105 | Protein S100-A12 | 116.4 | 16.1 | 121.4 | 14.7 | 93.3 | 17.4 | 30.3 | 5.0 | <.0001 |
P81265 | Polymeric immunoglobulin receptor | 112.9 | 12.4 | 122.5 | 13.5 | 80.9 | 6.6 | 40.6 | 4.8 | <.0001 |
P81644 | Apolipoprotein A-II | 120.1 | 13.0 | 128.3 | 14.9 | 102.2 | 7.1 | 85.2 | 8.8 | 0.041 |
Q0VCN9 | Folate receptor 2 (Fetal) | 150.4 | 11.0 | 121.9 | 13.7 | 78.1 | 4.3 | 43.3 | 3.1 | <.0001 |
Q17QG8 | Histone H2A | 81.5 | 27.4 | 105.7 | 15.1 | 96.6 | 21.2 | 29.6 | 5.7 | <.0001 |
Q1JPB0 | Leukocyte elastase inhibitor | 132.9 | 23.2 | 93.0 | 10.1 | 86.0 | 8.6 | 46.7 | 6.2 | <.0001 |
Q29S21 | Keratin, type II cytoskeletal 7 | 111.6 | 9.2 | 131.8 | 12.6 | 102.2 | 7.6 | 74.3 | 5.3 | <.0001 |
Q2KII3 | Hepatitis A virus cellular receptor 1 N-terminal domain containing protein | 127.3 | 9.2 | 116.9 | 13.3 | 105.8 | 7.6 | 68.3 | 3.9 | <.0001 |
Q3SYR8 | Immunoglobulin J chain | 124.3 | 8.8 | 111.7 | 7.8 | 76.4 | 4.2 | 42.9 | 4.8 | <.0001 |
Q3SZV7 | Hemopexin | 134.8 | 17.3 | 99.9 | 10.4 | 89.3 | 12.1 | 51.6 | 8.3 | <.0001 |
Q8SQ28 | Serum amyloid A protein | 123.8 | 11.6 | 133.5 | 22.9 | 109.2 | 11.4 | 67.5 | 9.7 | 0.001 |