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Fig. 2 | Journal of Animal Science and Biotechnology

Fig. 2

From: Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections

Fig. 2

Mitochondrial functions analysis in CCs and oocytes post heat stress. (a-b) Representative images and the ratios of different mitochondrial distribution patterns in oocytes; (c) mtDNA copy number of CCs and oocytes (n = 4); (d-e) Representative images and the content of mROS in CCs and oocytes (n = 30); (f-g) mtDNA-encoded genes expression levels in CCs and oocytes (n = 4). Data are expressed as mean ± SEM. Control, ovaries were kept in 38.5 °C water bath for 1 h, and then the isolated COCs were cultured in 38.5 °C for 24 h; Heat, ovaries were kept in 41.5 °C water bath for 1 h, and then COCs were isolated and cultured in 38.5 °C for another 24 h. *P < 0.05, **P < 0.01 and # 0.05 < P < 0.1, compared with control

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