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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Heat exposure impairs porcine oocyte quality with suppressed actin expression in cumulus cells and disrupted F-actin formation in transzonal projections

Fig. 1

Oocyte quality and cumulus expansion analysis under HS. (a) Oocyte survival rate and the first polar body extrusion rate after 1 h of ovarian HS (n = 4); (b) Evaluation of oocyte maturational progression after HS exposure (n = 30). (c-f) Representative images (magnification, × 10) and the statistical analysis of cumulus expansion patterns (c-d) and the survival rates of oocytes and CCs (e-f) at different stages during in vitro maturation after 1 h of 41.5 °C HS (n = 4). GV: Germinal vesicle; GVBD: Germinal vesicle break down; MI: First meiotic metaphase; TI: First meiotic telophase; MII: Second meiotic metaphase. Data are expressed as mean ± SEM. Control, ovaries were kept in 38.5 °C water bath for 1 h, and then the isolated COCs were cultured in 38.5 °C for another 8 h, 16 h, 24 h or 44 h; Heat, ovaries were kept in 41.5 °C water bath for 1 h, and then COCs were isolated and cultured in 38.5 °C for another 8 h, 16 h, 24 h or 44 h. *P < 0.05, **P < 0.01 and # 0.05 < P < 0.1, compared with control; a, b, c, mean values not sharing the same letters are significantly different, P < 0.05; ND, no detected

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