Table 1 Primers for real-time PCR analysesa
Gene | Primer sequence (5′ to 3′) | Accession number | Size, bp |
|---|---|---|---|
GAPDH | F:GGGTCATCATCTCTGCACCT | NM_001034034 | 176 |
R:GGTCATAAGTCCCTCCACGA | |||
IL-6 | F:TCCTTGCTGCTTTCACACTC | NM_173923.2 | 129 |
R:CACCCCAGGCAGACTACTTC | |||
IL-1β | F:CAGTGCCTACGCACATGTCT | NM_174093.1 | 209 |
R:AGAGGAGGTGGAGAGCCTTC | |||
TNF-α | F:GCCCTCTGGTTCAGACACTC | NM_173966.3 | 192 |
R:AGATGAGGTAAAGCCCGTCA | |||
TLR-2 | F:CAGGCTTCTTCTCTGTCTTGT | NM_174197.2 | 140 |
R:CTGTTGCCGACATAGGTGATA | |||
IL-8 | F:TGGGCCACACTGTGAAAAT | NM_173925.2 | 136 |
R:TCATGGATCTTGCTTCTCAGC | |||
κ-casein | F: CCAGGAGCAAAACCAAGAAC | NM_174294.2 | 148 |
R: TGCAACTGGTTTCTGTTGGT | |||
IL-10 | F:TGTATCCACTTGCCAACCAG | NM_174088.1 | 126 |
R:CAGCAGAGACTGGGTCAACA | |||
L-selectin | F:CTCTGCTACACAGCTTCTTGTAAACC | NM_174182.1 | 104 |
R:CCGTAGTACCCCAAATCACAGTT | |||
IRAK1 | F: CCTCAGCGACTGGACATCCT | NM_001040555.1 | 103 |
R:GGACGTTGGAACTCTTGACATCT | |||
NFKBIA | F:GGGAGACCTGGCCTTCCTC | NM 001045868.1 | 101 |
R:CCAGAAGTGCCTCAGCGATT | |||
TRAF6 | F:AGAACAGATGCCCAATCACTATGAT | NM_001034661.2 | 100 |
R:GTGATTCCTCTGCATCTTTTCATG | |||
Lysozyme | F:GAGAGATGTGAGCTTGCCAGAA | NM_001078159.1 | 120 |
R:TGTAGCTTGTGTGTTGTAATTGCTTTC | |||
SOD2 | F:GAGAAGGGTGATGTTACAGCTCAGA | NM_201527.2 | 100 |
R:GGCTCAGATTTGTCCAGAAGATG | |||
TLR-4 | F:GACCCTTGCGTACAGGTTGT | NM_174198.6 | 103 |
R:GGTCCAGCATCTTGGTTGAT | |||
β-actin | F:GACCCAGATCATGTTCGAGA | NM_173979.3 | 145 |
R:CTCATAGATGGGCACCGTGT |