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Table 3 Characteristics of the antibodies used for western blot analysisa

From: Protective effects of taurine against muscle damage induced by diquat in 35 days weaned piglets

Antibody

Catalog number

Source

Dilution rate

HSP70

ab5439

Mouse

1:2,000

MAFbx

55,456–1-AP

Rabbit

1:600

MuRF1

12,866–1-AP

Rabbit

1:500

GAPDH

10,494–1-AP

Rabbit

1:7,000

HRP goat anti-rabbit IgG

SA00001–2

Goat

1:6,000

HRP goat anti-mouse IgG

SA00001–2

Goat

1:5,000

  1. aHSP70, heat shock protein 70; MAFbx, muscle atrophy F-box; MuRF1, muscular ring finger protein 1; GAPDH, glyceraldehyde-3-phosphate dehydrogenase