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Table 1 Alpha diversity measurements of the ruminal microbiota

From: Dietary energy sources and levels shift the multi-kingdom microbiota and functions in the rumen of lactating dairy cows

Measurements LE HE SEM P-values
GC SFC GC SFC NE ES Period NE × ES
Bacteria and archaea
 Observed ASVs 1050 808 973 752 45.211 0.3456 0.0116 0.1646 0.7936
 Observed genera 110A 100AB 100AB 91B 2.738 0.0368 0.0756 0.3117 0.8548
 Observed species 126A 115AB 113AB 104B 3.127 0.0360 0.1006 0.3115 0.7053
 Shannon 8.27 8.24 8.22 8.00 0.063 0.2532 0.3962 0.8501 0.5160
 Simpson 0.99 0.99 0.99 0.99 0.001 0.7580 0.5245 <.0001 0.3247
 Faith’s PD 57.66 50.46 54.08 48.65 1.429 0.2187 0.0285 0.1718 0.5956
 Evenness 0.83 0.86 0.83 0.84 0.006 0.4262 0.1314 0.5571 0.4853
Fungi
 Observed ASVs 97AB 101A 71AB 68B 4.511 0.0008 0.9189 0.6974 0.7098
 Observed genera 50 52 41 40 1.947 0.0107 0.8837 0.7432 0.7498
 Observed species 63 64 47 49 2.931 0.0106 0.7737 0.8287 0.9804
 Shannon 5.33 5.46 4.91 5.03 0.075 0.0052 0.4194 0.3448 0.8850
 Simpson 0.95 0.96 0.94 0.95 0.003 0.0794 0.1340 0.1374 0.8218
 Faith’s PD 25.75 26.58 20.36 22.03 0.989 0.0128 0.4742 0.6513 0.7740
 Evenness 0.81 0.82 0.81 0.83 0.007 0.7033 0.2685 0.0047 0.7557
Protozoa
 Observed ASVs 73A 69AB 61AB 61B 2.275 0.0051 0.4157 0.6152 0.5865
 Observed genera 5 5 4 4 0.153 0.0131 0.6678 0.4967 0.3796
 Observed species 10A 9AB 8B 9AB 0.243 0.0046 0.7940 0.5114 0.2616
 Shannon 4.79 4.62 4.61 4.54 0.116 0.4194 0.4437 0.6601 0.7803
 Simpson 0.93 0.91 0.94 0.93 0.012 0.4435 0.5476 0.4481 0.5636
 Faith’s PD 0.87 0.82 0.72 0.76 0.024 0.0133 0.9700 0.7342 0.2230
 Evenness 0.77 0.76 0.78 0.77 0.015 0.7145 0.5960 0.5357 0.9009
  1. LE Low energy, HE High energy, GC Ground corn, SFC Steam-flaked corn, NE Effect of dietary energy level, ES Effect of energy sources, NE × ES Interaction between NE and ES
  2. ASVs Amplicon sequence variants
  3. Faith’s PD Faith’s phylogenetic diversity
  4. Different superscripts in upper case within a row indicate significant difference (P ≤ 0.05). The P-values ≤0.05 are bolded, while those > 0.05 but ≤0.1 are italicized