| Treatment2 |  SEM | P-value3 |
---|
 | LAA-C | LAA-GG | LAA-LG | HAA-C | HAA-GG | HAA-LG |  | GG | LG | AA | GG × AA | LG × AA |
---|
EIF2A | 1.08 | 0.95 | 1.04 | 1.16 | 0.87 | 1.06 | 0.207 | 0.12 | 0.59 | 0.81 | 0.54 | 0.78 |
EIF2AK3 | 1.09 | 1.05 | 1.25 | 1.66 | 1.54 | 1.39 | 0.240 | 0.70 | 0.80 | 0.15 | 0.85 | 0.31 |
EIF2B5 | 1.12ab | 0.91b | 1.04ab | 1.04ab | 1.07ab | 1.61a | 0.198 | 0.62 | 0.17 | 0.05 | 0.50 | 0.07 |
EIF4E | 1.29 | 0.97 | 1.40 | 1.40 | 1.16 | 1.57 | 0.271 | 0.18 | 0.49 | 0.37 | 0.84 | 0.86 |
PRKAA1 | 1.33 | 1.83 | 1.54 | 2.04 | 1.94 | 1.64 | 0.399 | 0.64 | 0.56 | 0.89 | 0.34 | 0.28 |
RPS6KB1 | 1.25 | 0.80 | 0.99 | 0.91 | 0.74 | 0.92 | 0.205 | 0.14 | 0.54 | 0.78 | 0.47 | 0.50 |
- a,bMeans within a row with no common superscripts differ (P ≤ 0.05)
- 1Values are least-squares means from milk fat collected on d 5 of each period. Fold changes in gene expression from RNA captured in milk fat globules relative to LAA-C were calculated by the 2-ΔΔCt method after normalizing to RPS9
- 2LAA-C, 0.9% saline; LAA-GG, 1319 g/d glucose; LAA-LG, 676 g/d palm olein; HAA-C, 844 g/d of a complete EAA mixture in the same profile and amount as found in 1.5 kg casein; HAA-GG, 1319 g/d glucose + 844 g/d EAA; HAA-LG, 676 g/d palm olein + 844 g/d EAA. Infusions of LAA-GG, LAA-LG, and HAA-C supplied 20.5 MJ/d, and HAA-GG and HAA-LG supplied 41.0 MJ/d. For all treatments n = 6
- 3GG = effect of energy from glucose; LG = effect of energy from fat; AA = effect of protein level