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Table 1 Primer sequences used for real-time quantitative PCR

From: Impact of post-ruminally infused macronutrients on bovine mammary gland expression of genes involved in fatty acid synthesis, energy metabolism, and protein synthesis measured in RNA isolated from milk fat

Gene1

Forward primer (5′→3′)

Reverse primer (5′→3′)

Accession No. reference

ACC1

GTGAAGTTCCCTCAGGCTCTTAATC

TGTCTGAGCAGATATCCACTTCC

NM_174224.2

CASP3

CAGCGTCGTAGCTGAACGTA

GTTTGCTGCATCCACGTCTG

NM_001077840

CCND1

GCTCCTGTGCTGCGAGATG

GCTCTTTTTCACGGGCTCCA

NM_001046273

CDC42BPA

ATGAAAAGGATGCACGAGGGT

GGCATATCTGTTGCTCGGGT

NM_001192937.2

CSN2

CAGGCCTTTCTGCTGTACCA

CAAAAGTGAGGAGGGGGCAT

KC993858

DDIT3

CAAAGCCGGAACCTGAGGAG

TCCTGCAGGTCCTCATACCA

NM_001078163

DDIT4

AGCCTTTGGGATCGCTTCTC

GCTGATTTGGGGTGGGAGTT

NM_001075922

EIF2A

TACAGAAACCATGCCCATCA

TGCAAGTTCGGTCTCATCTG

NM_175813

EIF2AK3

GGCTGAAAGATGACAGCACA

AGAACTGGCTCTCGGATGAA

NM_001098086

EIF2B5

ACTGACAAAGGCCAGCAGTT

GACGGTGGTCACTCATCCTT

XM_002684878

EIF4E

CAGTGCTGTGCCTTATTGGA

TGCATGGGACTGATAACCAA

NM_174310

FASN

CCAAGTCGAACATGGGACAT

GATCTTGGGGTTTGGGTTGT

NM_001012669.1

GAPDH

GGGTCATCATCTCTGCACCT

GGTCATAAGTCCCTCCACGA

NM_001034034

HSPA5

TGAACGACCCCTGACGAAAG

TGCGCTCCTTGAGCTTTTTG

NM_001075148

IDH1

ACACTGAGTGACTGTGTGCTC

CTTGGTGACCTGGTCGTTGG

NM_181012.3

LALBA

AGTCCTTTCGTCCCAGCACTA

AACCGGAGTCTGCTTGATGA

JN258330

ME2

GTTCTCCCCGGTCAGTCTCCT

TTTTCTCACCCCGCTTCTTGC

NM_001076814.1

MYC

GTAGTAATTCCAGCGAGAGGCA

TAGGCTAGCTCGGCTCTTCC

NM_001046074

PCK2

GCATCCCAACTCTCGCTTTTG

GGGGGACTCCTTTGGGTCT

NM_001205594.1

PPP1R15A

CAACCAGGAGACACAGAGGA

ACTCTGGGTTGAAGGGAGG

NM_001046178

PRKAA1

AGCCCTTCCTTCTCTTGCTC

AGGATGCCTGAAAAGCTTGA

NM_001109802

RPL15

ACACTATTGGTGGCTCTCGC

ACAAACATCACGTGTTAGCGG

XM_005226176

RPS6

TGTGCGAAAGCCCCTAAACA

GGAGTCACGAGACGCTGAAT

NM_001015548

RPS6KB1

TGACAGCCCAGATGACTCAG

TGGGCTGCCAATAATCTTC

NM_205816

RPS9

CTGAAGCTGATCGGCGAGTA

GGGTCTTTCTCATCCAGCGT

NM_001101152.2

UXT

CGCTACGAGGCTTTCATCTC

CCGAGTGGTTAGCTTCCTGG

NM_001037471.2

XBP1s

TGCTGAGTCCGCAGCAGGTG

AGAATGCCCAACAGGATGTC

BC102639

XBP1u

AGACTACGTGCACCTCTGCAG

AGAATGCCCAACAGGATGTC

BC102639

  1. 1ACC1 = acetyl-CoA carboxylase 1; CASP3 = caspase 3; CCND1 = cyclin D1; CDC42BPA = CDC42 binding protein kinase alpha; CSN2 = β-casein; DDIT3 = DNA damage-inducible transcript 3; DDIT4 = DNA damage inducible transcript 4; EIF2A = eukaryotic initiation factor 2, α subunit; EIF2AK3 = eukaryotic translation initiation factor 2, alpha kinase 3; EIF2B5 = eukaryotic translation initiation factor 2B, ε subunit; EIF4E = eukaryotic translation initiation factor 4E; FASN = fatty acid synthase; GAPDH = glyceraldehyde 3-phosphate dehydrogenase; HSPA5 = H3 histone, family 3A; IDH1 = isocitrate dehydrogenase 1, cytosolic; LALBA = α-lactalbumin; ME2 = malic enzyme 2, mitochondrial; MYC = MYC proto-oncogene; PCK2 = phosphoenolpyruvate carboxykinase 2, mitochondrial; PPP1R15A = protein kinase AMP-activated catalytic, α1 subunit; PRKAA1 = protein kinase AMP-activated catalytic, α1 subunit; RPL15 = ribosomal protein L15; RPS6 = ribosomal protein S6; RPS6KB1 = ribosomal protein S6 kinase B1; RPS9 = ribosomal protein S9; UXT = ubiquitously expressed prefoldin like chaperone; XBP1s = X-box binding protein 1, spliced; XBP1u = X-box binding protein 1, unspliced