Effect of dietary fiber and threonine content on intestinal barrier function in pigs challenged with either systemic E. coli lipopolysaccharide or enteric Salmonella Typhimurium

Table 1 Primers used in quantitative PCR analysis^a

Gene	Forward (5′ to 3′)	Reverse (5′ to 3′)	AT, °C	NCBI accession number
RPL19	AACTCCCGTCAGCAGATCC	AGTACCCTTCCGCTTACCG	60	AF_435591
MUC2	ACCCGCACTACGTCACCTTC	GGCAGGACACCTGGTCATTG	62	BX671371
CLDN4	CAACTGCGTGGATGATGAGA	CCAGGGGATTGTAGAAGTCG	60	NM_001161637.1
GAPDH	CTTCACGACCATGGAGAAGG	CCAAGCAGTTGGTGGTACAG	63	AF017079
ZO-1	ACGGCGAAGGTAATTCAGTG	CTTCTCGGTTTGGTGGTCTG	60	XM_003353439.2
IL8	TCCTGCTTTCTGCAGCTCTC	GGGTGGAAAGGTGTGGAATG	62	NM_213867
Casp3	AGAGGGGACTGCTGTAGAACT	CCGTCTCAATCCCACAGTCC	59	NM_214131.1

^aGAPDH glyceraldehyde 3-phosphate dehydrogenase, RPL19 ribosomal protein-L19, ZO1 Zonula Occludin −1,MUC2 Mucin-2, CLDN-4 Claudin-4, IL8 Interleukin-8, Casp3 Caspase-3, AT annealing temperature

ISSN: 2049-1891