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Fig. 1 | Journal of Animal Science and Biotechnology

Fig. 1

From: Effects of undigested protein-rich ingredients on polarised small intestinal organoid monolayers

Fig. 1

Experimental overview of the study. a Duodenum organoids were generated from 3 individual mice and separately cultured in Matrigel. b 3-dimensional organoids were single-cell dissociated by pipetting and TrypLE enzymatic digestion, plated in Matrigel coated 96-well plates, and grown until confluence for 3 d. c After confluence the monolayers were treated for 6 h with 4% w/v soybean meal (SBM), casein (CAS), spray-dried plasma protein (SDPP), yellow mealworm (YMW), or medium control (MC). d After 6 h, the monolayers were lysed and processed for gene expression profiling using Affymetrix microarrays

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