Table 1 Primer sequences for the RT-PCR and q-PCR analyses
From: Establishment of cell lines with porcine spermatogonial stem cell properties
Gene | Forward primer (5'→3') | Reverse primer (5'→3') | Product size, bp |
|---|---|---|---|
UCHL1 | CAGTAGCCAATAATCAGGAC | AAGGCATCCGACCATCAAG | 249 |
PLZF | GCGGAAGACCTGGATGACCT | GTCGTCTGAGGCTTGGATGGT | 105 |
THY1 | TACCACCAACCTGCCCATTC | AGAAGTTGGTTCGAGAGCGG | 116 |
VASA | TATGATGCGGGATGGAATAACTG | ACAGCTCTTACACAAGTCCCAA | 144 |
DAZL | CCTTTGCTGACCCACAGTCT | GTGAGGGACTGGCCAATGAA | 220 |
CXCR4 | TCTACGCTTTCCTTGGAGCC | GAAGAATGTCCACCCCGCTT | 115 |
SV40 | AGAACAGCCCAGCCACTATAA | CCAAGCAACTCCAGCCAT | 256 |
PCNA | GAACAGGAGTACAGCTGTGTA | CAGGCTCATTCATCTCTATGG | 220 |
GAPDH | TCGGAGTGAACGGATTTGGC | TGACAAGCTTCCCGTTCTCC | 189 |
HPRT1 | TTATGCCGAGGATTTGGAAAAGGTT | ATCCAGCAGGTCAGCAAAGAAT | 160 |
KIT | CATGCACCAATGAAGGCGGTT | CAGCCCGTGAGGGAGTAATT | 166 |
STRA8 | AGCCGTTTACTTTCACTCTGACC | GCTGTTTGCATTCCCATCCT | 148 |